Overview
TFE (Targeted Feature Extractor) is a fast, lightweight application for targeted extraction and manual review of LC-MS features from both HRAM and QQQ data. It was built to make feature review practical at large scale, with responsive visualization and interaction across more than 1,000 samples without major memory or performance limitations. If you know the m/z values and approximate retention times of your target features, TFE provides a simple and efficient way to extract, inspect, and manually refine them.
Users provide LC-MS files and an annotation list containing retention times, m/z values, and integration parameters. TFE extracts the target chromatograms and integrates peaks based on the parameters. At any time the user can update these values and reintegrate, manually change the integration bounds or even add new targets and samples.
Anyone is free to use and redistribute TFE in its original form. Modification and redistribution of modified versions requires permission from the Metabolomics Platform at the Broad Institute.
Quick Start
No installation is required. Simply download the appropriate release from the releases page and run the executable named tfe.
TFE is built in electron and cross-platform compatible. We currently have builds for x86-64 and ARM architectures on:
- Windows
- Linux
- macOS
For a quick tutorial and walkthrough video, download the example dataset below and watch the accompanying video demonstration, which covers most core functionality in TFE.
Video Under Construction