Waddington Optimal Transport (wot) uses time-course data to infer how the probability distribution of cells in gene-expression space evolves over time, by using the mathematical approach of optimal transport.

If you use this toolkit, please consider reading the citing wot section on how to properly cite it in your work

Command line

wot features a command line interface that offers fine-grained control over all calculations.

View documentation »


Classify your cells based on gene expression, identify new cell sets, compare cell populations with differential expression, track the evolution of a population over time, identify the shared ancestors of two populations, or isolate key marker genes.

View detailed examples »

Generic placeholder image

Open Source

The full source code for this package, the examples presented here, and archives with precomputed transport maps on simulated data, along with Python scripts for the most common use cases are available on Github.

View on Github »