StringTie2

Quantify

description
Quantify gene expression using StringTie2.

Inputs

Required

  • aligned_bai (File, required): Aligned reads in BAI format.
  • aligned_bam (File, required): Aligned reads in BAM format.
  • gtf (File, required): Reference GTF file.

Optional

  • runtime_attr_override (RuntimeAttr?): Override default runtime attributes.

Defaults

  • keep_retained_introns (Boolean, default=false): Keep retained introns.
  • prefix (String, default="out"): Prefix for output files.

Outputs

  • st_gtf (File)
  • st_abund (File)

ExtractTranscriptSequences

description
Extract transcript sequences from a GTF file.

Inputs

Required

  • gtf (File, required): Reference GTF file.
  • ref_fasta (File, required): Reference FASTA file.
  • ref_fasta_fai (File, required): Reference FASTA index file.

Optional

  • runtime_attr_override (RuntimeAttr?): Override default runtime attributes.

Defaults

  • prefix (String, default="out"): Prefix for output files.

Outputs

  • transcripts_fa (File)
  • transcripts_fai (File)
  • transcripts_dict (File)

CompareTranscriptomes

description
Compare two GTF files.

Inputs

Required

  • guide_gtf (File, required): Reference GTF file.
  • new_gtf (File, required): New GTF file.

Optional

  • runtime_attr_override (RuntimeAttr?): Override default runtime attributes.

Defaults

  • prefix (String, default="out"): Prefix for output files.

Outputs

  • annotated_gtf (File)
  • loci (File)
  • tracking (File)
  • refmap (File)
  • tmap (File)
  • stats (File)