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A set of command line tools (in Java) for manipulating high-throughput sequencing (HTS) data and formats such as SAM/BAM/CRAM and VCF.

View the Project on GitHub broadinstitute/picard

Decoding Base Qualities

This utility makes it easy to convert between base quality values encoded in ASCII (as recorded in a FASTQ or SAM file) and their integer values representing the Phred-scaled probability that each base call is correct (as explained here).

Select encoding scale:
Sanger Solexa Illumina 1.3+ Illumina 1.5+ Illumina 1.8+

See the FASTQ format documentation for more details on encoding scales.

Enter the ASCII string or Phred value you want to convert in the box below:
Multiple Phred values can be entered separated by commas.